Removal of polycyclic aromatic hydrocarbon (PAH)-contaminated sediments by persulfate oxidation and determination of degradation product cytotoxicity based on HepG2 and ZF4 cell lines.
Autor: Dong, Cheng-Di; Tsai, Mei-Ling; Wang, Tsing-Hai; Chang, Jih-Hsing; Chen, Chiu-Wen; Hung, Chang-Mao
Publication year: 2020
Environmental science and pollution research international
issn:1614-7499 0944-1344
doi: 10.1007/s11356-019-04421-w
Abstract:
This study evaluated the use of magnetite (Fe(3)O(4)), carbon black (CB), and Fe(3)O(4)-CB composites activated by persulfate (PS) at circumneutral pH to oxidize polycyclic aromatic hydrocarbons (PAHs) in marine sediments. In addition, the in vitro cytotoxic activity and apoptotic response of the obtained degradation products were investigated. Chemical analyses showed that the total PAH concentration was 26,263 ng/g for sediment samples from an industrial port area. Highly toxic BaP was the main contributor to the TEQ in sediments. Source analyses demonstrated that the PAHs in the sediment were derived from coal combustion. In this study, we found that the PS oxidation processes effectively degrade PAHs at concentration levels of 1.7 × 10(-5) M at pH 6.0. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was employed to assess the cytotoxicity of the PAH degradation products before and after Fe(3)O(4)/PS, CB/PS, and Fe(3)O(4)-CB/PS oxidation treatment using a human hepatoma carcinoma cell line (HepG2) and a zebrafish (Danio rerio) embryonic cell line (ZF4). Each sample extract showed a marked dose-related response, with the cell viability reduced by 82% in the case of HepG2 and 58% in the case of ZF4 at 100 μg/mL after the Fe(3)O(4)-CB/PS process. The PAH degradation products had different effects on the cell morphologies of the two cell lines. The results suggested that the ZF4 cell model is more sensitive than HepG2 to the toxicity of the PAH samples.
Language: eng
Rights:
Pmid: 30746626
Tags: Humans; Oxidation-Reduction; MTT assay; Cell Line; Cytotoxicity; Water Pollutants, Chemical/*analysis; Carbon black; Geologic Sediments; HepG2 cells; Magnetite; Persulfate; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons/*analysis; ZF4 cells
Link: https://pubmed.ncbi.nlm.nih.gov/30746626/
