Matrix-assisted ionization Fourier transform mass spectrometry for the analysis of lipids.
Autor: Liyanage, Rohana; Gidden, Jennifer; Wilkins, Charles L.; Lay, Jackson O. Jr
Publication year: 2021
Rapid communications in mass spectrometry : RCM
issn:1097-0231 0951-4198
doi: 10.1002/rcm.8349
Abstract:
RATIONALE: Assessing the utility of vacuum matrix-assisted ionization (MAI) for the direct and rapid analysis of lipids in complex samples with emphasis on bacterial taxonomy. METHODS: Matrix-assisted ionization Fourier transform mass spectrometry (MAI-FTMS) was used to characterize polar and non-polar lipids in mixtures. RESULTS: For non-polar lipid triacylglycerols (TAGs), MAI-FTMS produced lipid-specific ions for eight different edible oils and allowed these oils to be identified based on their MAI-FTMS profiles. For polar lipids from bacteria, MAI-FTMS of crude lipid extracts allowed taxonomic identification of eight blind-coded samples based on taxonomy-specific phospholipid profiles. MAI produced results comparable and complementary to benchmark MALDI and ESI methods currently used for characterization of polar and non-polar lipids in the same mixtures. CONCLUSIONS: The newly developed MAI technique is a rapid, simple and complementary method for the characterization of polar and non-polar lipids in complex mixtures.
Language: eng
Rights: © 2018 John Wiley & Sons, Ltd.
Pmid: 30421829
Tags: *Lipids/analysis/chemistry; Bacteria/chemistry; Fourier Analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
Link: https://pubmed.ncbi.nlm.nih.gov/30421829/
