The protective effect of Zataria multiflora Boiss essential oil on CCl(4) induced liver fibrosis in rats.
Autor: Barghi, Maryam; Ashrafi, Mahboobeh; Aminlari, Mahmoud; Namazi, Fateme; Nazifi, Saeed
Publication year: 2021
Drug and chemical toxicology
issn:1525-6014 0148-0545
doi: 10.1080/01480545.2019.1571502
Abstract:
Activation of hepatic stellate cells by free radicals is an initial step in the development of liver fibrosis. Zataria multiflora Boiss (ZM) essential oil as a natural product has antioxidant activity and maybe a suitable candidate for treatment or prevention of the disease. Thus, this study aims to evaluate the protective effect of ZM oil in CCl(4) induced liver fibrosis. Male rats were divided into 5 groups, group C: control rats; CO: vehicle control group; CE: rats that received essential oil (500 µl/kg); F: fibrosis group, rat were intraperitoneally injected with CCl(4) (1 mL/kg); FE: fibrosis rats that received both CCl(4) and ZM essential oil as mentioned above. At the end of the 11th week, serum samples and liver tissues were collected for the evaluation of fibrosis markers, liver enzymes, oxidative stress parameters and histopathological studies. The results showed a significant increase in the activity of serum AST, ALT, total bilirubin, TGF-β1, hyaluronan, and hydroxyproline levels in serum and liver tissues in F group. Also, an abnormality in lipid profile and the existence of oxidative stress was found in serum and liver tissues in F group compared to the control groups. Our study showed that ZM essential oil could ameliorate mentioned parameters. Histopathological examinations confirmed the results of biochemical evaluations.
Language: eng
Rights:
Pmid: 30746963
Tags: Male; Animals; Rats; Rats, Sprague-Dawley; Oxidative Stress/*drug effects; carbon tetrachloride; essential oil; Lamiaceae/*chemistry; Antioxidants/isolation & purification/pharmacology; Carbon Tetrachloride/toxicity; rat; Hepatic Stellate Cells/drug effects/metabolism; Liver Cirrhosis/*prevention & control; liver fibrosis; Oils, Volatile/isolation & purification/*pharmacology; Zataria multiflora Boiss
Link: https://pubmed.ncbi.nlm.nih.gov/30746963/
