The cytotoxic and genotoxic effects of daidzein on MIA PaCa-2 human pancreatic carcinoma cells and HT-29 human colon cancer cells.
Autor: Gundogdu, Gulsah; Dodurga, Yavuz; Cetin, Meltem; Secme, Mucahit; Cicek, Betul
Publication year: 2020
Drug and chemical toxicology
issn:1525-6014 0148-0545
doi: 10.1080/01480545.2018.1527849
Abstract:
Daidzein (DZ) has anti-inflammatory and antioxidant effects, as well as the dose-dependent inhibition effect on cancer cells. In this study, the cytotoxic and genotoxic effects of DZ on HT-29 (human colorectal adenocarcinoma cells) and MIA PaCa-2 (human pancreatic cancer cells) cell lines were determined using the XTT method and Comet assay, respectively. IC(50) concentrations of DZ were found to be 200 µM in both MIA PaCa-2 and HT-29 cells treated with DZ for 48 hours (h). When the cells were treated with 200 μM of DZ for 48 h, DNA damage was observed in both cell lines. DNA tail length (TL), tail moment (TM), and tail intensity (TI) increased more in MIA PaCa-2 cells treated with 200 μM of DZ than those in the control cell (untreated MIA PaCa-2 cell) group (p < 0.01). However, only DNA-TI and DNA-TM exhibited higher increases in HT-29 cells treated with 200 μM of DZ than those in the control cell (untreated HT-29 cell) group (p < 0.01). This shows that DZ has cytotoxic and genotoxic effects on both cell lines. The observed genotoxic effects of DZ still need to be confirmed in additional future studies. Language: eng Rights: Pmid: 30394111 Tags: Humans; Inhibitory Concentration 50; Dose-Response Relationship, Drug; Cell Survival/drug effects; *DNA Damage; Comet Assay; comet assay; Antineoplastic Agents, Phytogenic/*pharmacology; Cancer cells; Carcinoma/*drug therapy/genetics/pathology; Colonic Neoplasms/*drug therapy/genetics/pathology; cytotoxic and genotoxic effect; daidzein; HT29 Cells; Isoflavones/*pharmacology; Pancreatic Neoplasms/*drug therapy/genetics/pathology; XTT Link: https://pubmed.ncbi.nlm.nih.gov/30394111/
