Phytochemical characterization and mutagenicity, cytotoxicity, antimicrobial and modulatory activities of Poincianella pyramidalis (Tul.) L.P. Queiroz.
Autor: Chaves, Thiago P.; Medeiros, Francinalva D.; Sousa, João Marcelo C.; Silva, Luís Augusto P.; Lima, Micheline A.; Coutinho, Henrique D. M.; Medeiros, Ana Cláudia D.
Publication year: 2020
Natural product research
issn:1478-6427 1478-6419
doi: 10.1080/14786419.2019.1566724
Abstract:
In this study, the chemical composition, genotoxic, cytotoxic and antibacterial-modulating activities of the P. pyramidalis (NPpE) extract was evaluated. The fingerprint chromatogram was determined using HPLC-DAD. The NPpE Minimal Inhibitory Concentration (MIC), as well as that of antimicrobial drugs in the presence and absence of the extract, were evaluated using the microdilution method against Gram positive bacteria. In vivo assays with mice were used for the determination of the extract’s genotoxicity and cytotoxicity. The presence of the polyphenol catechin was confirmed in the extract. The extract showed significant antimicrobial activity (MIC ≤ 1000 µg mL(-1)) against Staphylococcus aureus, Streptococcus oralis and S. mutans. When the NPpE was associated with several antimicrobials, the MIC of most of these were significantly reduced (P < 0.001) demonstrating good prospective usage in antimicrobial therapy. The extract has mutagenic and cytotoxic potential, however, further studies should be performed to confirm their toxicity. Language: eng Rights: Pmid: 30689403 Tags: Animals; Mice; Microbial Sensitivity Tests; catechin; genotoxicity; cytotoxicity; Mutagenicity Tests; Drug Evaluation, Preclinical; Anti-Bacterial Agents/chemistry/*pharmacology; Chromatography, High Pressure Liquid; Staphylococcus aureus/drug effects; Plant Extracts/chemistry/pharmacology; Fabaceae/*chemistry; HPLC-DAD; Polyphenols/analysis; Catechin/pharmacology; Phytochemicals/analysis/chemistry/*pharmacology/toxicity; Poincianella pyramidalis; Streptococcus oralis/drug effects Link: https://pubmed.ncbi.nlm.nih.gov/30689403/
