Light directed migration of a cluster of cells in the centimeter scale.
Autor: Mosabbir, Abdullah Al; Truong, Kevin
Publication year: 2020
Small GTPases
issn:2154-1256 2154-1248
doi: 10.1080/21541248.2017.1396390
Abstract:
Protein-based systems for light directed migration of cells have been demonstrated up to distances of several hundred microns, but larger distances in the centimeter scale would allow new possible applications. Light activated migration in mammalian cells can be achieved by cells expressing channelrhodopsin-2 and an engineered Ca(2+) sensitive Rac1 protein called RACer. In this study, light was used to induce wound healing, localize cells into a region of interest, and move cells over centimeter scale distances. Given the spatially complex organization of different types of cells in real tissue, light directed migration over the centimeter scale could potentially organize cell type arrangement to help develop more realistic tissues for transplantation.
Language: eng
Rights:
Pmid: 29173049
Tags: Humans; Cells, Cultured; Cluster Analysis; Rac1; *Cell Engineering; *Light; Ca2+ signalling; Cell Movement; channelrhodopsin-2; engineered stable cell line; HEK293 Cells; Light directed migration; Luminescent Proteins/*genetics; Wound Healing/*genetics
Link: https://pubmed.ncbi.nlm.nih.gov/29173049/
