In vitro evaluation of biological activities of Orobanche crenata Forssk. leaves extract.
Autor: Genovese, Carlo; Acquaviva, Rosaria; Ronsisvalle, Simone; Tempera, Gianna; Antonio Malfa, Giuseppe; D’Angeli, Floriana; Ragusa, Salvatore; Nicolosi, Daria
Publication year: 2020
Natural product research
issn:1478-6427 1478-6419
doi: 10.1080/14786419.2018.1552697
Abstract:
Orobanche crenata Forssk. (Orobanchaceae) is a holoparasitic plant noxious to legumes. Orobanchaceae are well known in traditional medicine for their hypotensive, antihyperglycemic, antispasmodic, analgesic and antimicrobial effects. In this work, the biological activities of the acetonic leaves extract were evaluated. Qualitative chemical analysis was determined through thin layer chromatography. Total phenols and flavonoids content was obtained by the Folin-Ciocalteu and the Aluminium chloride colorimetric methods, respectively. The antimicrobial activity was performed by the broth microdilution method. The extract showed antibacterial activity, particularly against Gram-positive strains, with MIC ranging from 376.00 to 3011.00 µg/ml. Besides, it exhibited antifungal effects on Candida spp. with MIC ranging from 94.10 to 3011.00 µg/ml. In addition, O. crenata inhibited superoxide anion formation and showed a DPPH quenching capacity, particularly at 80 µg/ml. Interestingly, our results are promising thus indicating the importance of O. crenata as a significant source of biologically active natural compounds.
Language: eng
Rights:
Pmid: 30672323
Tags: Microbial Sensitivity Tests; Flavonoids/analysis; Plant Leaves/chemistry; antioxidant activity; Gram-Negative Bacteria/drug effects; antimicrobial activity; Phenols/analysis; polyphenols; Anti-Bacterial Agents/pharmacology; Candida/drug effects; Antioxidants/chemistry/pharmacology; Antifungal Agents/pharmacology; Orobanche crenata; Orobanche/*chemistry; Plant Extracts/*chemistry/*pharmacology; Superoxides/chemistry/metabolism
Link: https://pubmed.ncbi.nlm.nih.gov/30672323/
