Gypensapogenin H from hydrolyzate of total Gynostemma pentaphyllum saponins induces apoptosis in human breast carcinoma cells.
Autor: Zhang, Xiaoshu; Shi, Guohui; Wu, Xiaojun; Zhao, Yuqing
Publication year: 2020
Natural product research
issn:1478-6427 1478-6419
doi: 10.1080/14786419.2018.1525370
Abstract:
Gypensapogenin H (Gyp H) is a novel dammarane-type triterpene, isolated from hydrolyzate of total saponins from Gynostemma pentaphyllum. Our previous work demonstrated that Gyp H exhibited potent growth inhibitory effects on tumor cells. It significantly inhibited the growth of human breast cancer cells (MDA-MB-231), while having low toxicity to normal human breast epithelial cells, MCF-10a. Further mechanistic study demonstrated that Gyp H decreased survival, inhibited proliferation, migration, induced apoptosis and led to cell cycle arrest. For the MDA-MB-231 cell lines, Gyp H increased expression of P21, Bax and cytochrome c, induced PARP cleavage and activated caspases. Gyp H also reduced expression of CDK2/4, CyclinD1, E2F1 and Bcl2, which associated with the cell cycle arrest. Thus, our finding may be useful for understanding the mechanism of action of Gyp H on breast cancer cells and suggest that Gyp H would be a leading agent for the treatment of breast cancer.
Language: eng
Rights:
Pmid: 30470142
Tags: Humans; Female; Cell Line, Tumor; apoptosis; Cell Proliferation/drug effects; Apoptosis/drug effects; Plant Extracts/chemistry/pharmacology; Hydrolysis; Antineoplastic Agents, Phytogenic/chemistry/*pharmacology; Breast Neoplasms/*drug therapy/metabolism/pathology; cell cycle block; Cell Cycle Checkpoints/drug effects/physiology; Cell Movement/drug effects; Cyclin D1/metabolism; Cytochromes c/pharmacology; Dammaranes; Gynostemma pentaphyllum; Gynostemma/*chemistry; New dammarane triterpene; Proto-Oncogene Proteins c-bcl-2/metabolism; Saponins/chemistry/*pharmacology; Triterpenes/chemistry
Link: https://pubmed.ncbi.nlm.nih.gov/30470142/
