Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs.
Autor: Chen, Peng; Kang, Tao-Tao; Bao, Xi-Yan; Dong, Zhan-Qi; Zhu, Yan; Xiao, Wen-Fu; Pan, Min-Hui; Lu, Cheng
Publication year: 2020
Insect science
issn:1744-7917 1672-9609
doi: 10.1111/1744-7917.12664
Abstract:
As an important insect immune response, apoptosis plays a critical role in the interaction between baculoviruses and insect hosts. Previous reports have identified inhibitor of apoptosis (IAP) proteins in both insects and baculoviruses, but the relationship between these proteins is still not clearly understood. Here, we found that insect IAP proteins were clustered with baculovirus IAP3, suggesting that the baculovirus iap3 gene might be derived from the Lepidoptera or Diptera. We demonstrated that Bombyx mori inhibitor of apoptosis (Bmiap) gene had an inhibitory effect on apoptosis in silkworm cells. Further analysis of the effects of Bmiap genes on the proliferation of B. mori nucleopolyhedrovirus (BmNPV) showed that both the Bmiap and BmNPV iap genes increased BmNPV proliferation after BmNPV infected silkworm cells. Our results also indicated that BmNPV IAP1 and IAP2 directly interacted with BmIAP in silkworm cells, implying that the Bmiap gene might be hijacked by BmNPV iap genes during BmNPV infection. Taken together, our results provide important insights into the functional relationships of iap genes, and improve our knowledge of apoptosis in baculoviruses and insect hosts.
Language: eng
Rights: © 2019 Institute of Zoology, Chinese Academy of Sciences.
Pmid: 30697933
Tags: Animals; Biological Evolution; Phylogeny; Bombyx mori; evolution; Cell Line; Insect Proteins/genetics/metabolism; *Nucleopolyhedroviruses/growth & development/metabolism; Apoptosis/genetics; baculovirus; baculovirus-host interaction; BmNPV; Bombyx/metabolism/virology; Host Microbial Interactions; inhibitor of apoptosis; Inhibitor of Apoptosis Proteins/*genetics/metabolism; Viral Proteins/*genetics/metabolism
Link: https://pubmed.ncbi.nlm.nih.gov/30697933/
