Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin.
Autor: Nna, Victor Udo; Bakar, Ainul Bahiyah Abu; Ahmad, Azlina; Mohamed, Mahaneem
Publication year: 2020
Archives of physiology and biochemistry
issn:1744-4160 1381-3455
doi: 10.1080/13813455.2018.1543329
Abstract:
Context: Metformin’s effect on glycaemic control is well documented, but its effect on diabetes-induced testicular impairment has been scarcely reported.Objective: To investigate the effects of metformin on testicular oxidative stress, inflammation, and apoptosis, which largely contribute to fertility decline in diabetic state.Methods: Male Sprague-Dawley rats were divided into 3 groups (n = 6/group) namely: normal control (NC), diabetic control (DC), and metformin (300 mg/kg b.w./d)-treated diabetic groups. Metformin was administrated for 4 weeks.Results: Decreased mRNA expressions and activities of antioxidant enzymes were seen in the testes of DC group. mRNA and protein expressions of pro-inflammatory and pro-apoptotic markers increased, while interleukin-10 and proliferating cell nuclear antigen (PCNA) decreased in the testes of DC group. Treatment with metformin up-regulated antioxidant enzymes, down-regulated inflammation, and apoptosis and increased PCNA immunoexpression in the testes.Conclusions: Metformin protects the testes from diabetes-induced impairment and may improve male reproductive health in diabetic state.
Language: eng
Rights:
Pmid: 30513216
Tags: Male; Animals; inflammation; oxidative stress; Rats; Rats, Sprague-Dawley; Oxidative Stress/*drug effects; apoptosis; Apoptosis/*drug effects; Diabetes mellitus; metformin; Diabetes Mellitus, Experimental/*complications; Hypoglycemic Agents/*pharmacology; Inflammation/*drug therapy/etiology/metabolism/pathology; Interleukin-10/metabolism; Metformin/*pharmacology; Proliferating Cell Nuclear Antigen/metabolism; Protective Agents/*pharmacology
Link: https://pubmed.ncbi.nlm.nih.gov/30513216/
