Calprotectin instability may lead to undertreatment in children with IBD.
Autor: Haisma, Sjoukje-Marije; van Rheenen, Patrick Ferry; Wagenmakers, Lucie; Muller Kobold, Anneke
Publication year: 2020
Archives of disease in childhood
issn:1468-2044 0003-9888
doi: 10.1136/archdischild-2018-316584
Abstract:
BACKGROUND: Treatment decisions in children with inflammatory bowel disease (IBD) are increasingly based on longitudinal tracking of faecal calprotectin concentrations, but there is little known about the stability of this protein in stool. METHODS: We stored aliquots of homogenised stool at room temperature and at 4°C, and measured the calprotectin concentration for 6 consecutive days with three different assays. In addition, we assessed calprotectin stability in assay-specific extraction buffers kept at room temperature. RESULTS: After 6 days of storage at room temperature, mean percentage change from baseline calprotectin concentrations in stool and extraction buffer was 35% and 46%, respectively. The stability of calprotectin was significantly better preserved in samples stored at 4°C (p=0.0066 and 0.0011, respectively). CONCLUSIONS: Calprotectin is not stable at room temperature. Children with IBD and their caretakers may be falsely reassured by low calprotectin values. The best advisable standard for preanalytical calprotectin handling is refrigeration of the stool sample until delivery at the hospital laboratory.
Language: eng
Rights: © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
Pmid: 30655264
Tags: Humans; Child; Temperature; Biomarkers/metabolism; monitoring; *Specimen Handling; Feces/*chemistry; gastroenterology; Inflammatory Bowel Diseases/*metabolism; Leukocyte L1 Antigen Complex/*metabolism; Protein Stability
Link: https://pubmed.ncbi.nlm.nih.gov/30655264/
